Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Neuronal UCP1 expression suggests a mechanism for local thermogenesis during hibernation.

Authors
  • Laursen, Willem J
  • Mastrotto, Marco
  • Pesta, Dominik
  • Funk, Owen H
  • Goodman, Jena B
  • Merriman, Dana K
  • Ingolia, Nicholas
  • Shulman, Gerald I
  • Bagriantsev, Sviatoslav N
  • Gracheva, Elena O
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Feb 03, 2015
Volume
112
Issue
5
Pages
1607–1612
Identifiers
DOI: 10.1073/pnas.1421419112
PMID: 25605929
Source
Medline
Keywords
License
Unknown

Abstract

Hibernating mammals possess a unique ability to reduce their body temperature to ambient levels, which can be as low as -2.9 °C, by active down-regulation of metabolism. Despite such a depressed physiologic phenotype, hibernators still maintain activity in their nervous systems, as evidenced by their continued sensitivity to auditory, tactile, and thermal stimulation. The molecular mechanisms that underlie this adaptation remain unknown. We report, using differential transcriptomics alongside immunohistologic and biochemical analyses, that neurons from thirteen-lined ground squirrels (Ictidomys tridecemlineatus) express mitochondrial uncoupling protein 1 (UCP1). The expression changes seasonally, with higher expression during hibernation compared with the summer active state. Functional and pharmacologic analyses show that squirrel UCP1 acts as the typical thermogenic protein in vitro. Accordingly, we found that mitochondria isolated from torpid squirrel brain show a high level of palmitate-induced uncoupling. Furthermore, torpid squirrels during the hibernation season keep their brain temperature significantly elevated above ambient temperature and that of the rest of the body, including brown adipose tissue. Together, our findings suggest that UCP1 contributes to local thermogenesis in the squirrel brain, and thus supports nervous tissue function at low body temperature during hibernation.

Report this publication

Statistics

Seen <100 times