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Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1.

Authors
  • Breslin, Mary B1
  • Wang, Hong-Wei
  • Pierce, Amy
  • Aucoin, Rebecca
  • Lan, Michael S
  • 1 The Research Institute for Children, Children's Hospital in New Orleans, 200 Henry Clay Avenue, Research and Education Building, Rm. 2211, New Orleans, LA 70118, USA.
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Mar 06, 2007
Volume
581
Issue
5
Pages
949–954
Identifiers
PMID: 17300785
Source
Medline
Language
English
License
Unknown

Abstract

INSM1 is a downstream target gene of neurogenin 3 (ngn3). A promoter construct containing the -426/+40bp region transiently co-transfected into NIH-3T3 cells with a ngn3 expression plasmid resulted in a 12-fold increase in promoter activity. The ngn3/E47 heterodimer selectively binds and activates the E-box3 of the INSM1 promoter. The endogenous ngn3 and CREB-binding protein (CBP) co-activator occupy the INSM1 promoter, resulting in hyper-acetylation of histone H3/H4 chromatin in a human neuroblastoma cell line, IMR-32. Additionally, adenoviral ngn3 can induce endogenous INSM-1 expression in pancreatic ductal carcinoma-1 cells through the recruitment of CBP to the INSM1 promoter and increase the acetylation of the INSM1 promoter region.

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