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Neurogenesis of adipose-derived stem cells in hydrogel

Authors
  • Xie, Xuewei1, 2
  • Tang, Zhouping1
  • Chen, Juan1
  • Yang, Jie1
  • Zeng, Wengao1
  • Liu, Na1
  • Liu, Yongming1
  • 1 Tongji Medical University, Huazhong University of Science and Technology, Department of Neurology, Tongji Hospital, Wuhan, 430030, China , Wuhan (China)
  • 2 Changzheng Hospital, the Second Military Medical University, Department of Neurology, Shanghai, 200003, China , Shanghai (China)
Type
Published Article
Journal
Journal of Huazhong University of Science and Technology [Medical Sciences]
Publisher
Huazhong University of Science and Technology
Publication Date
Apr 20, 2011
Volume
31
Issue
2
Pages
174–177
Identifiers
DOI: 10.1007/s11596-011-0246-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.

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