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Neuregulin induces CTGF expression in hypertrophic scarring fibroblasts.

Authors
  • Kim, Jun-Sub
  • Choi, Ihn-Geun
  • Lee, Boung-Chul
  • Park, Jae-Bong
  • Kim, Jin-Hee
  • Jeong, Je Hoon
  • Jeong, Ji Hoon
  • Seo, Cheong Hoon
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Jun 01, 2012
Volume
365
Issue
1-2
Pages
181–189
Identifiers
DOI: 10.1007/s11010-012-1258-2
PMID: 22350758
Source
Medline
License
Unknown

Abstract

Hypertrophic scarring (HTS) is a common fibroproliferative disorder that typically follows thermal and other injuries involving the deep dermis. These pathogenic mechanisms are regulated by connective tissue growth factor (CTGF) and transforming growth factor-β. We found that neuregulin-1 (NRG1), as well as NRG receptors, HER-2, and HER-3 were upregulated in HTS fibroblasts (HTSF), compared with normal fibroblasts. Furthermore, NRG1 stimulation increased the expression of CTGF in HTSF. In the presence of inhibitors of PI3K, Src, Smad, or reactive oxygen species, the effect of NRG1 on CTGF expression decreased significantly. In particular, the combination of LY294002 or PP2 with SB431542 blocked NRG1-mediated CTGF expression in HTSF. Finally, we demonstrated that siRNA for CTGF, AG825, LY294002, and PP2, either alone or in co-treatment, effectively reduced extracellular matrix expression. Taken together, our results suggest that NRG1 is involved in fibrotic scar pathogenesis via PI3K- or Src-mediated CTGF expression.

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