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A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

Authors
  • Brandon-Mong, Guo-Jie1, 2, 3
  • Shaw, Grace Tzun-Wen1
  • Chen, Wei-Hsin4
  • Chen, Chien-Chang4, 5
  • Wang, Daryi1, 3
  • 1 Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei, 11529, Taiwan , Taipei (Taiwan)
  • 2 National Taiwan Normal University, Taipei, Taiwan , Taipei (Taiwan)
  • 3 Biodiversity Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan Normal University, Taipei, Taiwan , Taipei (Taiwan)
  • 4 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan , Taipei (Taiwan)
  • 5 Taiwan International Graduate Program in Molecular Medicine, National Yang-Ming University, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei, 11529, Taiwan , Taipei (Taiwan)
Type
Published Article
Journal
BMC Microbiology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 30, 2020
Volume
20
Issue
1
Identifiers
DOI: 10.1186/s12866-020-01981-7
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundNeuropathic pain is an abnormally increased sensitivity to pain, especially from mechanical or thermal stimuli. To date, the current pharmacological treatments for neuropathic pain are still unsatisfactory. The gut microbiota reportedly plays important roles in inducing neuropathic pain, so probiotics have also been used to treat it. However, the underlying questions around the interactions in and stability of the gut microbiota in a spared nerve injury-induced neuropathic pain model and the key microbes (i.e., the microbes that play critical roles) involved have not been answered. We collected 66 fecal samples over 2 weeks (three mice and 11 time points in spared nerve injury-induced neuropathic pain and Sham groups). The 16S rRNA gene was polymerase chain reaction amplified, sequenced on a MiSeq platform, and analyzed using a MOTHUR- UPARSE pipeline.ResultsHere we show that spared nerve injury-induced neuropathic pain alters gut microbial diversity in mice. We successfully constructed reliable microbial interaction networks using the Metagenomic Microbial Interaction Simulator (MetaMIS) and analyzed these networks based on 177,147 simulations. Interestingly, at a higher resolution, our results showed that spared nerve injury-induced neuropathic pain altered both the stability of the microbial community and the key microbes in a gut micro-ecosystem. Oscillospira, which was classified as a low-abundance and core microbe, was identified as the key microbe in the Sham group, whereas Staphylococcus, classified as a rare and non-core microbe, was identified as the key microbe in the spared nerve injury-induced neuropathic pain group.ConclusionsIn summary, our results provide novel experimental evidence that spared nerve injury-induced neuropathic pain reshapes gut microbial diversity, and alters the stability and key microbes in the gut.

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