Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Negative visualization of DNA in agarose gel by eosin Y and its related mechanism.

Authors
Type
Published Article
Journal
The Analyst
1364-5528
Publisher
The Royal Society of Chemistry
Publication Date
Volume
137
Issue
6
Pages
1466–1472
Identifiers
DOI: 10.1039/c2an00008c
PMID: 22310316
Source
Medline
License
Unknown

Abstract

We report here a sensitive and environmentally benign technique for the visualization of DNA by using eosin Y, which appeared as a transparent and colorless band under the opaque gel matrix background. As low as 0.2-0.6 ng of DNA can be visualized within 1 h, similar to that of the most commonly used ethidium bromide stain. Furthermore, the mechanism of the newly developed method was also described. According to the results, it can be concluded that the complex between DNA and eosin Y is mainly formed by the function of groove binding, but not intercalate itself into the double helix structure of DNA.

Statistics

Seen <100 times