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Negative ion chemical ionization mass spectrometry of 2,4-dinitrophenyl amino acid esters

Authors
  • Williams, Todd D.
  • Vachon, Lori
  • Anderegg, Robert J.
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1986
Volume
153
Issue
2
Pages
372–379
Identifiers
DOI: 10.1016/0003-2697(86)90105-3
Source
Elsevier
Keywords
License
Unknown

Abstract

We report the negative ion chemical ionization mass spectra of 2,4-dinitrophenyl (DNP) amino acid methyl esters. For the common amino acids, these derivatives exhibit very simple mass spectra; the molecular anion is the base peak in all cases. The electrophilicity of the DNP group allows selective and sensitive ionization. Amino acids can be identified singly or in mixtures by their molecular weights, and picomole detection is possible. Chromatography is only needed for Leu Ile differentiation. Amino-terminal analysis of proteins is demonstrated.

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