Affordable Access

The need for modification of the polyvinyl sponge model of connective tissue growth. Histoligic and biochemical studies in the rabbit.

Authors
Type
Published Article
Journal
Connective tissue research
Publication Date
Volume
3
Issue
4
Pages
213–225
Identifiers
PMID: 126135
Source
Medline
License
Unknown

Abstract

Quantitative histopathologic and biochemical comparisons were made between polyvinyl sponge capsular and sponge tissue in the rabbit on different days after subcutaneous implantation. Up to 9 days the predominant cell type in the capsular tissue is the fibroblast and in the sponge it is the neutrophil. During this time period the sponge tissue shows lower rates of (14C) proline and (14C) cytidine incorporation and lower rates of total (14C) collagen synthesis than the surrounding capsule. Different gel electrophoretic patterns of isolated radioactive proteins are found in sponge and capsule at 6 days. These biochemical differences appear to be related to the small number of fibroblasts, relative to granulocytes present in sponges during the first 9 days after implantation. It is suggested that future biochemical investigations of the early phase of connective tissue reactions (first 9 days) in this model utilize sponge capsular tissue within 1 cm of the sponge edge instead of the sponge and its contents.

Statistics

Seen <100 times