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Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies.

Authors
  • Richie, Christopher T1
  • Whitaker, Leslie R1
  • Whitaker, Keith W2
  • Necarsulmer, Julie1
  • Baldwin, Heather A1
  • Zhang, Yajun3
  • Fortuno, Lowella1
  • Hinkle, Josh J1
  • Koivula, Pyry1
  • Henderson, Mark J1
  • Sun, Wenzhi4
  • Wang, Kai4
  • Smith, Jeffrey C5
  • Pickel, Jim6
  • Ji, Na4
  • Hope, Bruce T1
  • Harvey, Brandon K7
  • 1 Intramural Research Program, National Institute on Drug Abuse, Baltimore, MD 21224, United States. , (United States)
  • 2 Intramural Research Program, National Institute on Drug Abuse, Baltimore, MD 21224, United States; US Army Research Laboratory, Aberdeen Proving Ground, MD 21005, United States. , (United States)
  • 3 Intramural Research Program, National Institute on Drug Abuse, Baltimore, MD 21224, United States; Intramural Research Program, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852, United States. , (United States)
  • 4 Janelia Research Campus,Howard Hughes Medical Institute, Ashburn, VA 20147, United States. , (United States)
  • 5 Intramural Research Program, National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892, United States. , (United States)
  • 6 Intramural Research Program, National Institute of Mental Health, Bethesda, MD 20892, United States. , (United States)
  • 7 Intramural Research Program, National Institute on Drug Abuse, Baltimore, MD 21224, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Journal of neuroscience methods
Publication Date
Jun 01, 2017
Volume
284
Pages
1–14
Identifiers
DOI: 10.1016/j.jneumeth.2017.03.020
PMID: 28380331
Source
Medline
Keywords
License
Unknown

Abstract

Overall, we have demonstrated that iRFP713 can be used as a reporter in neurons without the use of exogenous biliverdin, with minimal impact on viability and function thereby making it feasible to extend the capabilities for imaging genetically-tagged neurons in slices and in vivo.

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