Affordable Access

Access to the full text

Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei

Authors
  • Behera, Soumya P.1
  • Dubey, Abhinav1, 2
  • Chen, Wan-Na1
  • De Paula, Viviane S.3
  • Zhang, Meng1
  • Sgourakis, Nikolaos G.3
  • Bermel, Wolfgang4
  • Wagner, Gerhard1
  • Coote, Paul W.1, 2
  • Arthanari, Haribabu1, 2
  • 1 Harvard Medical School, Boston, MA, 02115, USA , Boston (United States)
  • 2 Dana-Farber Cancer Institute, Boston, MA, 02215, USA , Boston (United States)
  • 3 University of California, Santa Cruz, CA, 95064, USA , Santa Cruz (United States)
  • 4 Bruker BioSpin GmbH, Rheinstetten, 76287, Germany , Rheinstetten (Germany)
Type
Published Article
Journal
Nature Communications
Publisher
Springer Nature
Publication Date
Nov 03, 2020
Volume
11
Issue
1
Identifiers
DOI: 10.1038/s41467-020-19325-4
Source
Springer Nature
License
Green

Abstract

The structure and dynamics of large proteins and complexes can be studied by methyl-NMR but resonance assignment is still challenging. Here, the authors present a NMR method that leverages optimal control pulse design to unambiguously distinguish between Leu and Val using a simple 2D HMQC experiment and they apply it to several proteins including Cas9, interleukin, and human translation initiation factor eIF4a.

Report this publication

Statistics

Seen <100 times