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The Naturally Evolved EPSPS From Goosegrass Confers High Glyphosate Resistance to Rice

Authors
  • Ouyang, Chao1
  • Liu, Wei2
  • Chen, Silan1
  • Zhao, Huimin1
  • Chen, Xinyan1
  • Jin, Xiongxia1
  • Li, Xinpeng1
  • Wu, Yongzhong1
  • Zeng, Xiang1
  • Huang, Peijin1
  • He, Xiuying2
  • An, Baoguang1
  • 1 Hainan Bolian Rice Gene Technology Co., Ltd., Haikou , (China)
  • 2 Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangzhou , (China)
Type
Published Article
Journal
Frontiers in Plant Science
Publisher
Frontiers Media SA
Publication Date
Oct 29, 2021
Volume
12
Identifiers
DOI: 10.3389/fpls.2021.756116
Source
Frontiers
Keywords
Disciplines
  • Plant Science
  • Original Research
License
Green

Abstract

Glyphosate-resistant crops developed by the CP4-EPSPS gene from Agrobacterium have been planted on a massive scale globally, which benefits from the high efficiency and broad spectrum of glyphosate in weed control. Some glyphosate-resistant (GR) genes from microbes have been reported, which might raise biosafety concerns. Most of them were obtained through a hygromycin-HPT transformation system. Here we reported the plant source with 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene from goosegrass endowed rice with high resistance to glyphosate. The integrations and inheritability of the transgenes in the rice genome were investigated within two generations. The EiEPSPS transgenic plants displayed similar growth and development to wild type under no glyphosate selection pressure but better reproductive performance under lower glyphosate selection pressure. Furthermore, we reconstructed a binary vector pCEiEPSPS and established the whole stage glyphosate selection using the vector. The Glyphosate-pCEiEPSPS selection system showed a significantly higher transformation efficiency compared with the hygromycin-HPT transformation system. Our results provided a promising alternative gene resource to the development of GR plants and also extended the plant transformation toolbox.

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