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Nano/microformulations for Bacteriophage Delivery.

Authors
  • Cortés, Pilar1
  • Cano-Sarabia, Mary2
  • Colom, Joan3
  • Otero, Jennifer1
  • Maspoch, Daniel2, 4
  • Llagostera, Montserrat5
  • 1 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Spain. , (Spain)
  • 2 Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus de Bellaterra, Cerdanyola del Vallès, Spain. , (Spain)
  • 3 Deerland Ireland R&D Ltd., Food Science Building, University College Cork, Cork, Ireland. , (Ireland)
  • 4 ICREA, Barcelona, Spain. , (Spain)
  • 5 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Campus de Bellaterra, Cerdanyola del Vallès, Spain. [email protected]. , (Spain)
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2024
Volume
2734
Pages
117–130
Identifiers
DOI: 10.1007/978-1-0716-3523-0_7
PMID: 38066365
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials: a lipid cationic mixture and a combination of alginate with the antacid CaCO3. To perform bacteriophage encapsulation is necessary to dispose of a purified and highly concentrated lysate (around 1010 to 1011 pfu/mL) and a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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