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NAD(P)-dependent glucose dehydrogenase: Applications for biosensors, bioelectrodes, and biofuel cells.

Authors
  • Stolarczyk, Krzysztof1
  • Rogalski, Jerzy2
  • Bilewicz, Renata3
  • 1 Faculty of Chemistry, University of Warsaw, Pasteura St. 1, 02-093 Warsaw, Poland. , (Poland)
  • 2 Department of Biochemistry and Biotechnology, Maria Curie-Sklodowska University, Akademicka Str. 19, 20-031 Lublin, Poland. , (Poland)
  • 3 Faculty of Chemistry, University of Warsaw, Pasteura St. 1, 02-093 Warsaw, Poland. Electronic address: [email protected] , (Poland)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
May 23, 2020
Volume
135
Pages
107574–107574
Identifiers
DOI: 10.1016/j.bioelechem.2020.107574
PMID: 32498025
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This review discusses the physical and chemical properties of nicotinamide redox cofactor dependent glucose dehydrogenase (NAD(P) dependent GDH) and its extensive application in biosensors and bio-fuel cells. GDHs from different organisms show diverse biochemical properties (e.g., activity and stability) and preferences towards cofactors, such as nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+). The (NAD(P)+) play important roles in biological electron transfer, however, there are some difficulties related to their application in devices that originate from their chemical properties and labile binding to the GDH enzyme. This review discusses the electrode modifications aimed at immobilising NAD+ or NADP+ cofactors and GDH at electrodes. Binding of the enzyme was achieved by appropriate protein engineering techniques, including polymerisation, hydrophobisation or hydrophilisation processes. Various enzyme-modified electrodes applied in biosensors, enzymatic fuel cells, and biobatteries are compared. Importantly, GDH can operate alone or as part of an enzymatic cascade, which often improves the functional parameters of the biofuel cell or simply allows use of cheaper fuels. Overall, this review explores how NAD(P)-dependent GDH has recently demonstrated high potential for use in various systems to generate electricity from biological sources for applications in implantable biomedical devices, wireless sensors, and portable electronic devices. Copyright © 2020 Elsevier B.V. All rights reserved.

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