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NAD+ precursor increases aerobic performance in mice.

Authors
  • Crisol, Barbara M1
  • Veiga, Camilla B2
  • Braga, Renata R1
  • Lenhare, Luciene1, 3
  • Baptista, Igor L4
  • Gaspar, Rafael C1
  • Muñoz, Vitor R1
  • Cordeiro, André V1
  • da Silva, Adelino S R5, 6
  • Cintra, Dennys E2
  • Moura, Leandro P1, 7
  • Pauli, José R1, 7
  • Ropelle, Eduardo R8, 9, 10
  • 1 Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Pedro Zaccaria, 1300, Limeira, SP, 13484-350, Brazil. , (Brazil)
  • 2 Laboratory of Nutritional Genomics (LabGeN), School of Applied Sciences, University of Campinas, Limeira, SP, Brazil. , (Brazil)
  • 3 Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. , (Brazil)
  • 4 Laboratory of Cell and Tissue Biology, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil. , (Brazil)
  • 5 Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto, SP, Brazil. , (Brazil)
  • 6 Medical School, and Postgraduate Program in Physical Education and Sport, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. , (Brazil)
  • 7 CEPECE-Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, Brazil. , (Brazil)
  • 8 Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Pedro Zaccaria, 1300, Limeira, SP, 13484-350, Brazil. [email protected] , (Brazil)
  • 9 Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. [email protected] , (Brazil)
  • 10 CEPECE-Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, Brazil. [email protected] , (Brazil)
Type
Published Article
Journal
European Journal of Nutrition
Publisher
Springer-Verlag
Publication Date
Sep 01, 2020
Volume
59
Issue
6
Pages
2427–2437
Identifiers
DOI: 10.1007/s00394-019-02089-z
PMID: 31494696
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nicotinamide riboside (NR) acts as a potent NAD+ precursor and improves mitochondrial oxidative capacity and mitochondrial biogenesis in several organisms. However, the effects of NR supplementation on aerobic performance remain unclear. Here, we evaluated the effects of NR supplementation on the muscle metabolism and aerobic capacity of sedentary and trained mice. Male C57BL/6 J mice were supplemented with NR (400 mg/Kg/day) over 5 and 10 weeks. The training protocol consisted of 5 weeks of treadmill aerobic exercise, for 60 min a day, 5 days a week. Bioinformatic and physiological assays were combined with biochemical and molecular assays to evaluate the experimental groups. NR supplementation by itself did not change the aerobic performance, even though 5 weeks of NR supplementation increased NAD+ levels in the skeletal muscle. However, combining NR supplementation and aerobic training increased the aerobic performance compared to the trained group. This was accompanied by an increased protein content of NMNAT3, the rate-limiting enzyme for NAD + biosynthesis and mitochondrial proteins, including MTCO1 and ATP5a. Interestingly, the transcriptomic analysis using a large panel of isogenic strains of BXD mice confirmed that the Nmnat3 gene in the skeletal muscle is correlated with several mitochondrial markers and with different phenotypes related to physical exercise. Finally, NR supplementation during aerobic training markedly increased the amount of type I fibers in the skeletal muscle. Taken together, our results indicate that NR may be an interesting strategy to improve mitochondrial metabolism and aerobic capacity.

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