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NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor.

Authors
  • Wilkinson, Adam
  • Sayer, Heather
  • Bullard, Desmond
  • Smith, Andrew
  • Day, Jonathan
  • Kieser, Tobias
  • Bowater, Richard
Type
Published Article
Journal
Proteins Structure Function and Bioinformatics
Publisher
Wiley (John Wiley & Sons)
Publication Date
May 15, 2003
Volume
51
Issue
3
Pages
321–326
Identifiers
PMID: 12696044
Source
Medline
License
Unknown

Abstract

Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD(+)-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD(+) as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD(+)-dependent DNA ligases.

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