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N-acetylcysteine delivery with silica nanoparticles into 3T3-L1 adipocytes.

Authors
  • Frontera, Evelyn1
  • Desimone, Martin F2
  • Marzi, Mauricio C De1, 3
  • Guerra, Liliana N1, 3, 4
  • 1 Universidad Nacional de Luján. Departamento de Ciencias Básicas, Av Constitución y Ruta 5, Luján, Buenos Aires 6700, Argentina. , (Argentina)
  • 2 CONICET- Universidad de Buenos Aires. Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Buenos Aires, Argentina. , (Argentina)
  • 3 CONICET- Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable (INEDES), Laboratorio de Inmunología. Av. Constitución y Ruta 5, Luján, Buenos Aires 6700, Argentina. , (Argentina)
  • 4 Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica, Buenos Aires, Argentina. , (Argentina)
Type
Published Article
Journal
Therapeutic Delivery
Publisher
"Future Science, LTD"
Publication Date
Mar 17, 2021
Identifiers
DOI: 10.4155/tde-2020-0093
PMID: 33726506
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Background: The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials & methods: Silica NPs, 20 ± 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin. Results: While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.

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