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N,N'-Bis(4-azidobenzoyl)cystine--a cleavable photoaffinity reagent.

Authors
  • Ultee, M E
  • Basch, R S
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Sep 01, 1985
Volume
149
Issue
2
Pages
331–338
Identifiers
PMID: 4073491
Source
Medline
License
Unknown

Abstract

A water-soluble, cleavable, heterobifunctional photoaffinity label has been synthesized in one step from N-hydroxysuccinimidyl 4-azidobenzoate and cystine. The resultant compound, N,N'-bis(4-azidobenzoyl) cystine [(ABC)2], reacts with protein sulfhydryl groups through disulfide exchange to generate photoactive derivatives. Since [35S]cystine of high specific activity is readily available, it is possible to produce highly radioactive (ABC)2. ABC-derivatized ovalbumin is antigenic in vivo, and monoclonal antibodies specific for ABC have been produced. The antigen binding site of these antibodies was covalently labeled with ABC.

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