Affordable Access

deepdyve-link
Publisher Website

Myosin 1b flattens and prunes branched actin filaments.

Authors
  • Pernier, Julien1, 2, 3
  • Morchain, Antoine1, 2
  • Caorsi, Valentina4
  • Bertin, Aurélie1, 2
  • Bousquet, Hugo2, 3
  • Bassereau, Patricia5, 2
  • Coudrier, Evelyne6, 3
  • 1 Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France. , (France)
  • 2 Sorbonne Université, 75005 Paris, France. , (France)
  • 3 Laboratory Cell Biology and Cancer, Institut Curie, PSL Research University, C.N.R.S. UMR 144, 26 rue d'Ulm, Paris, France. , (France)
  • 4 Abbelight, 191 Avenue Aristide Briand, 94230 Cachan, France. , (France)
  • 5 Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005 Paris, France [email protected] [email protected] , (France)
  • 6 Sorbonne Université, 75005 Paris, France [email protected] [email protected] , (France)
Type
Published Article
Journal
Journal of Cell Science
Publisher
The Company of Biologists
Publication Date
Sep 24, 2020
Volume
133
Issue
18
Identifiers
DOI: 10.1242/jcs.247403
PMID: 32895245
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Motile and morphological cellular processes require a spatially and temporally coordinated branched actin network that is controlled by the activity of various regulatory proteins, including the Arp2/3 complex, profilin, cofilin and tropomyosin. We have previously reported that myosin 1b regulates the density of the actin network in the growth cone. Here, by performing in vitro F-actin gliding assays and total internal reflection fluorescence (TIRF) microscopy, we show that this molecular motor flattens (reduces the branch angle) in the Arp2/3-dependent actin branches, resulting in them breaking, and reduces the probability of new branches forming. This experiment reveals that myosin 1b can produce force sufficient enough to break up the Arp2/3-mediated actin junction. Together with the former in vivo studies, this work emphasizes the essential role played by myosins in the architecture and dynamics of actin networks in different cellular regions.This article has an associated First Person interview with the first author of the paper. © 2020. Published by The Company of Biologists Ltd.

Report this publication

Statistics

Seen <100 times