Affordable Access

Mycobacterium bovis: polymerase chain reaction identification in bovine lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism.

Authors
  • Zanini, M S
  • Moreira, E C
  • Lopes, M T
  • Oliveira, R S
  • Leão, S C
  • Fioravanti, R L
  • Roxo, E
  • Zumarraga, M
  • Romano, M I
  • Cataldi, A
  • Salas, C E
Type
Published Article
Journal
Memórias do Instituto Oswaldo Cruz
Publisher
SciELO
Publication Date
Aug 01, 2001
Volume
96
Issue
6
Pages
809–813
Identifiers
PMID: 11562707
Source
Medline
License
Unknown

Abstract

Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes.

Report this publication

Statistics

Seen <100 times