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The Myb-like transcription factor phosphorus starvation response (PtPSR) controls conditional P acquisition and remodelling in marine microalgae.

Authors
  • Kumar Sharma, Amit1
  • Mühlroth, Alice1
  • Jouhet, Juliette2
  • Maréchal, Eric2
  • Alipanah, Leila1
  • Kissen, Ralph1
  • Brembu, Tore3
  • Bones, Atle M1
  • Winge, Per1
  • 1 Cell, Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, 7491, Trondheim, Norway. , (Norway)
  • 2 Laboratoire de Physiologie Cellulaire Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Institut National de la Recherche Agronomique, Université Grenoble Alpes, 38000, Grenoble, France. , (France)
  • 3 Department of Biotechnology and Food Science, Norwegian University of Science and Technology, 7491, Trondheim, Norway. , (Norway)
Type
Published Article
Journal
New Phytologist
Publisher
Wiley (Blackwell Publishing)
Publication Date
Oct 09, 2019
Identifiers
DOI: 10.1111/nph.16248
PMID: 31598973
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Phosphorus (P) is one of the limiting macronutrients for algal growth in marine environments. Microalgae have developed adaptation mechanisms to P limitation that involve remodelling of internal phosphate resources and accumulation of lipids. Here, we used in silico analyses to identify the P-stress regulator PtPSR (Phaeodactylum tricornutum phosphorus starvation response) in the diatom P. tricornutum. ptpsr mutant lines were generated using gene editing and characterised by various molecular, genetics and biochemical tools. PtPSR belongs to a clade of Myb transcription factors that are conserved in stramenopiles and distantly related to plant P-stress regulators. PtPSR bound specifically to a conserved cis-regulatory element found in the regulatory region of P-stress-induced genes. ptpsr knockout mutants showed reduction in cell growth under P limitation. P-stress responses were impaired in ptpsr mutants compared with wild-type, including reduced induction of P-stress response genes, near to complete loss of alkaline phosphatase activity and reduced phospholipid degradation. We conclude that PtPSR is a key transcription factor influencing P scavenging, phospholipid remodelling and cell growth in adaptation to P stress in diatoms. © 2019 The Authors New Phytologist © 2019 New Phytologist Trust.

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