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Mutations of the RAG3 gene encoding a regulator of fermentation in Kluyveromyces lactis are suppressed by a mutation of the transcription factor gene KlGCR1.

Authors
  • Tizzani, Lorenza
  • Wésolowski-Louvel, Micheline
  • Forte, Valentina
  • Romitelli, Federica
  • Salani, Francesca
  • Lemaire, Marc
  • Neil, Helen
  • Bianchi, Michele M
Type
Published Article
Journal
FEMS yeast research
Publication Date
Aug 01, 2007
Volume
7
Issue
5
Pages
675–682
Identifiers
PMID: 17559574
Source
Medline
License
Unknown

Abstract

In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative-deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.

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