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Mutations in the catalase-peroxidase gene from isoniazid-resistant Mycobacterium tuberculosis isolates.

Authors
  • Altamirano, M
  • Marostenmaki, J
  • Wong, A
  • FitzGerald, M
  • Black, W A
  • Smith, J A
Type
Published Article
Journal
The Journal of infectious diseases
Publication Date
May 01, 1994
Volume
169
Issue
5
Pages
1162–1165
Identifiers
PMID: 8169415
Source
Medline
License
Unknown

Abstract

Isoniazid resistance in Mycobacterium tuberculosis has been associated with total deletion of the katG gene, which codes for catalase-peroxidase production. To determine whether this is a common mechanism of drug resistance, 9 isolates of isoniazid-resistant and 1 of isoniazid-sensitive M. tuberculosis were analyzed by polymerase chain reaction amplification of a 237-bp sequence of the katG gene. Amplification was observed in the isoniazid-sensitive isolate and in 8 resistant isolates; in only 1 isoniazid-resistant isolate was there no amplification of the expected band, suggesting gene deletion. DNA sequencing showed that 8 of the 9 isolates had point mutations, deletions, or insertions of 1-3 bases. Evidence corroborating the presence of mutations in the katG gene was obtained by single-strand conformation polymorphism analysis in these 8 isolates. Thus, mutations as well as insertions and deletions in the katG gene can account for inactive catalase peroxidase, leading to isoniazid resistance; gene deletion occurs only infrequently, in approximately 11% of cases.

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