Affordable Access

Mutation of an arginine biosynthesis gene causes reduced pathogenicity in Fusarium oxysporum f. sp. melonis.

Authors
  • Namiki, F
  • Matsunaga, M
  • Okuda, M
  • Inoue, I
  • Nishi, K
  • Fujita, Y
  • Tsuge, T
Type
Published Article
Journal
Molecular Plant-Microbe Interactions
Publisher
Scientific Societies
Publication Date
Apr 01, 2001
Volume
14
Issue
4
Pages
580–584
Identifiers
PMID: 11310747
Source
Medline
License
Unknown

Abstract

Restriction enzyme-mediated integration (REMI) mutagenesis was used to tag genes required for pathogenicity of Fusarium oxysporum f. sp. melonis. Of the 1,129 REMI transformants tested, 13 showed reduced pathogenicity on susceptible melon cultivars. One of the mutants, FMMP95-1, was an arginine auxotroph. Structural analysis of the tagged site in FMMP95-1 identified a gene, designated ARG1, which possibly encodes argininosuccinate lyase, catalyzing the last step for arginine biosynthesis. Complementation of FMMP95-1 with the ARG1 gene caused a recovery in pathogenicity, indicating that arginine auxotrophic mutation causes reduced pathogenicity in this pathogen.

Report this publication

Statistics

Seen <100 times