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Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium spp with zoonotic potential and an overview of human and animal infections.

Authors
  • Torres, Luciene de Fátima Costa1
  • Ribeiro, Dayana
  • Hirata, Raphael Jr
  • Pacheco, Luis Gustavo Carvalho
  • Souza, Monica Cristina
  • dos Santos, Louisy Sanches
  • dos Santos, Cíntia Silva
  • Salah, Mohammad
  • Costa, Mateus Matiuzzi da
  • Ribeiro, Marcio Garcia
  • Selim, Salah A
  • Azevedo, Vasco Ariston de Carvalho
  • Mattos-Guaraldi, Ana Luiza
  • 1 Universidade do Estado do Rio de Janeiro, Faculdade de Ciências Médicas, Laboratório de Difteria e Corinebactérias de Importância Clínica, Rio de Janeiro, RJ, Brasil.
Type
Published Article
Journal
Memorias do Instituto Oswaldo Cruz
Publication Date
May 01, 2013
Volume
108
Issue
3
Identifiers
DOI: 10.1590/S0074-02762013000300003
PMID: 23778659
Source
Medline
License
Unknown

Abstract

Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal and human hosts. Currently, there is a lack of information on the prevalence of disease caused by these pathogens, which is partially due to a reduction in the frequency of routine laboratory testing. In this study, a multiplex polymerase chain reaction (mPCR) assay that can simultaneously identify and determine the toxigenicity of these corynebacterial species with zoonotic potential was developed. This assay uses five primer pairs targeting the following genes: rpoB (Corynebacterium spp), 16S rRNA (C. ulcerans and C. pseudotuberculosis), pld (C. pseudotuberculosis), dtxR (C. diphtheriae) and tox [diphtheria toxin (DT) ]. In addition to describing this assay, we review the literature regarding the diseases caused by these pathogens. Of the 213 coryneform strains tested, the mPCR results for all toxigenic and non-toxigenic strains of C . diphtheriae, C. ulcerans and C. pseudotuberculosis were in 100% agreement with the results of standard biochemical tests and PCR-DT. As an alternative to conventional methods, due to its advantages of specificity and speed, the mPCR assay used in this study may successfully be applied for the diagnosis of human and/or animal diseases caused by potentially toxigenic corynebacterial species.

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