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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex.

Authors
  • Spositto, F L E
  • Campanerut, P A Z
  • Ghiraldi, L D
  • Leite, C Q F
  • Hirata, M H
  • Hirata, R D C
  • Siqueira, V L D
  • Cardoso, R Fressatti
Type
Published Article
Journal
Brazilian Journal of Microbiology
Publisher
Elsevier
Publication Date
Jan 01, 2014
Volume
45
Issue
3
Pages
841–843
Identifiers
PMID: 25477916
Source
Medline
Keywords
License
Unknown

Abstract

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

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