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Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome.

Authors
  • Hewitt, Chelsee Ann
  • Carmichael, Catherine Louise
  • Wilkins, Ella Jane
  • Cannon, Ping Zhi Fang
  • Pritchard, Melanie April
  • Scott, Hamish Steele
Type
Published Article
Journal
Frontiers in Bioscience
Publisher
Frontiers in BioScience
Publication Date
Jan 01, 2007
Volume
12
Pages
3010–3016
Identifiers
PMID: 17485278
Source
Medline
License
Unknown

Abstract

The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.

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