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Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.

Authors
  • Stephen, Laurie1
  • Schwarz, Emanuel2
  • Guest, Paul C3
  • 1 Ampersand Biosciences, 3 Main Street, Saranac Lake, NY, USA. [email protected]
  • 2 Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. , (Germany)
  • 3 Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil. , (Brazil)
Type
Published Article
Journal
Advances in experimental medicine and biology
Publication Date
Jan 01, 2017
Volume
974
Pages
149–156
Identifiers
DOI: 10.1007/978-3-319-52479-5_10
PMID: 28353231
Source
Medline
Keywords
License
Unknown

Abstract

Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labour than in single immunoassays. This chapter details the methods to develop and manufacture a 5-plex immunoassay for the Luminex® platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. An example will be given for the analysis of five hormones (glucagon-like peptide 1, growth hormone, insulin, leptin and thyroid-stimulating hormone) in serum samples from schizophrenia patients and controls.

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