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A multiplex fluorescent in situ hybridization protocol for clonal analysis of Drosophila oogenesis.

Authors
Type
Published Article
Journal
Methods in Molecular Biology
1064-3745
Publication Date
Volume
1189
Pages
115–122
Identifiers
DOI: 10.1007/978-1-4939-1164-6_8
PMID: 25245690
Source
Medline

Abstract

Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.

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