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Multiplex cytokine and antibody profile in cystic echinococcosis patients during a three-year follow-up in reference to the cyst stages

Authors
  • Li, Zhi-Dan1
  • Mo, Xiao-Jin1
  • Yan, Shuai1
  • Wang, Dong2
  • Xu, Bin1
  • Guo, Jian1
  • Zhang, Ting1, 3, 4
  • Hu, Wei1, 3
  • Feng, Yu2
  • Zhou, Xiao-Nong1
  • Feng, Zheng1
  • 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai, 200025, People’s Republic of China , Shanghai (China)
  • 2 Gansu Province Center for Disease Control and Prevention, Lanzhou, Gansu, 730020, People’s Republic of China , Lanzhou (China)
  • 3 Fudan University, Shanghai, 200438, People’s Republic of China , Shanghai (China)
  • 4 Xizang Center for Disease Control and Prevention, 21 Linkuo North Road, Lhasa, Tibet Autonomous Region, 850000, People’s Republic of China , Lhasa (China)
Type
Published Article
Journal
Parasites & Vectors
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Mar 14, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13071-020-4003-9
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundCystic echinococcosis (CE) is a worldwide parasitic zoonosis caused by infection of the larval stage of tapeworm Echinococcus granulosus. In human CE, the parasites develop and form cysts in internal organs. The differentiated cysts can be classified into five types based on WHO-IWGE standard CE1-5 representing different developmental stages. Infection with E. granulosus triggers hosts’ humoral and cellular response, displaying elevated serum antibodies and Th1 and Th2 cytokines, which are presumed to be in association with the disease outcome. Identification of immunological markers for evaluation of disease progression has been a growing concern. However, the distinctive profile of cytokines and antibodies associated with the cyst progression has not been ascertained.MethodsTo better understand the interaction between host immune response and disease outcome, the present study followed-up four CE patients over three years by yearly measuring serum level of 27 cytokines, total IgG and isotypes, and ultrasound scanning, beginning in year 1 for all patients with CE1 and CE2 cysts before treatment and continued in year 2 with CE4 and in year 3 with CE3-CE5 post-treatment.ResultsNine cytokines including Th1-type IL-2, Th17-type IL-17A, and inflammatory cytokines IL-1β, IL-1Rα and TNF-α, chemokines IL-8, MIP-1α, MIP-1β, and growth factor G-CSF were significantly elevated in patients with cyst type CE1, compared to the normal controls, and then declined to a normal level at CE4 and CE5. Examining the antibody production, we found that serum specific IgG was significantly increased in patients with active and transitional cysts, specifically the total IgG at CE1/CE3/CE4-CE5, IgG4 at CE1 and IgG1 at CE1/CE3 cyst status, in comparison with the normal controls, but showed no significant changes between the cyst stages.ConclusionsOur findings provide new information on the profile of multiplex cytokines and serum antibodies associated with cyst stages in cystic echinococcosis patients through a three-year follow-up, implying that further studies using an approach combining cyst-associated immune parameters may aid in identifying immunological markers for differentiation of disease progression.

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