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Multiple wavelength fluorescence enhancement on glass substrates for biochip and cell analyses.

Authors
  • Fouqué, B
  • Schaack, B
  • Obeïd, P
  • Combe, S
  • Gétin, S
  • Barritault, P
  • Chaton, P
  • Chatelain, F
Type
Published Article
Journal
Biosensors and Bioelectronics
Publisher
Elsevier
Publication Date
May 15, 2005
Volume
20
Issue
11
Pages
2335–2340
Identifiers
PMID: 15797336
Source
Medline
License
Unknown

Abstract

In microarrays experiments, a serious limitation is the unreliability of low signal intensities data and the lack of reproducibility for the resulting ratios between samples and controls. Most of the light emitted by a fluorophore at the air/glass interface of a glass slide is absorbed by the glass so just a part of the emitted fluorescence is detected. To improve the sensitivity of the fluorescence detection of both common fluorophores Cy3 and Cy5 in DNA microarrays and fluorescent cell analyses, we have designed a multi layer mirror with alternative thin layers of SiO2 and HfO2. This mirror (MOTL) prevents fluorescence absorption, allows the simultaneous enhancement of the fluorescence signals and increases the dynamic range of the slides. Using MOTL slides, Cy3 and Cy5 intensities are enhanced by 5-8-fold, consequently, the fluorescence analysis becomes easier and should allow the detection of low copy number genes or weakly fluorescent cells. With the same approach, other multiple optical thin layer slides could be designed for other series of fluorophores, extending the field of their applications.

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