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Multiple nucleosome positioning with unique rotational phasing on multimers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes: comparison between experimental and theoretical mapping.

Authors
  • Alessi, R
  • Cacchione, S
  • De Santis, P
  • Fuà, M
  • Savino, M
Type
Published Article
Journal
Biophysical Chemistry
Publisher
Elsevier
Publication Date
Sep 01, 1997
Volume
67
Issue
1-3
Pages
151–158
Identifiers
PMID: 9397524
Source
Medline
License
Unknown

Abstract

Nucleosome positioning along two DNA tracts, corresponding to tetramers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes, were studied by experimental (exonuclease III mapping and band shift electrophoresis) as well as theoretical methods. Multiple nucleosome positioning with unique rotational phase was derived from both methods in satisfactorily good agreement, if nucleosome dyad axis positions are considered. Theoretical and experimental distributions of nucleosome frequencies appear different, probably on account of DNA sequence dependent digestion kinetics of exonuclease III.

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