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A multi-omics concentration-response framework uncovers novel understanding of triclosan effects in the chlorophyte Scenedesmus vacuolatus.

Authors
  • Larras, Floriane1
  • Billoir, Elise2
  • Scholz, Stefan3
  • Tarkka, Mika4
  • Wubet, Tesfaye5
  • Delignette-Muller, Marie-Laure6
  • Schmitt-Jansen, Mechthild7
  • 1 Helmholtz-Centre for Environmental Research - UFZ, Department of Bioanalytical Ecotoxicology, Permoserstrasse 15, 04318 Leipzig, Germany. Electronic address: [email protected] , (Germany)
  • 2 Université de Lorraine, CNRS, LIEC, F-57000 Metz, France. , (France)
  • 3 Helmholtz-Centre for Environmental Research - UFZ, Department of Bioanalytical Ecotoxicology, Permoserstrasse 15, 04318 Leipzig, Germany. , (Germany)
  • 4 Department of Soil Ecology, Helmholtz-Centre for Environmental Research - UFZ, Theodor-Lieser-Straße 4, 06120 Halle, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany. , (Germany)
  • 5 Department of Community Ecology, Helmholtz-Centre for Environmental Research - UFZ, Theodor-Lieser-Straße 4, 06120 Halle, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany. , (Germany)
  • 6 Université de Lyon, Université Lyon 1, CNRS, VetAgro Sup, UMR 5558, Laboratoire de Biométrie et Biologie Evolutive, 69622 Villeurbanne, France. , (France)
  • 7 Helmholtz-Centre for Environmental Research - UFZ, Department of Bioanalytical Ecotoxicology, Permoserstrasse 15, 04318 Leipzig, Germany. Electronic address: [email protected] , (Germany)
Type
Published Article
Journal
Journal of hazardous materials
Publication Date
Apr 18, 2020
Volume
397
Pages
122727–122727
Identifiers
DOI: 10.1016/j.jhazmat.2020.122727
PMID: 32361673
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In aquatic ecosystems, the biocide triclosan represents a hazard for the non-target microalgae. So far, algal responses were mainly investigated at apical levels hampering the acquisition of a holistic view on primary, adaptive, and compensatory stress responses. We assessed responses of the chlorophyte Scenedesmus vacuolatus to triclosan at apical (growth, photosynthesis) and molecular (transcriptome, metabolome) levels for comparative pathway sensitivity analysis. For each responsive signal (contigs, metabolites), a concentration-response curve was modeled and effect concentrations were calculated leading to the setting of cumulative sensitivity distributions. Molecular responses showed higher sensitivity than apical observations. The functional annotation of contigs and metabolites revealed 118 metabolic pathways putatively impaired by triclosan, highlighting a wide repercussion on the algal metabolism. Metabolites involved in the lipid metabolism showed decreasing trends along the concentration gradient and a globally highest sensitivity, pointing to the primary target of triclosan. The pathways involved in xenobiotic degradation and membrane transporters were mainly regulated in the transcriptome with increasing response trends comprising compensatory responses. The suggested novel approach, combining apical and multi-omics analyses in a concentration-response framework improves mechanistic understanding and mode of action analysis on non-targeted organisms and is suggested to better implement high-throughput multi-omics data in environmental risk assessment. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

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