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Multilocus genotyping of Giardia duodenalis from pigs in Korea.

Authors
  • Lee, Haeseung1
  • Jung, ByeongYeal2
  • Lim, Jun-Sik3
  • Seo, Min-Goo2
  • Lee, Seung-Hun4
  • Choi, Kwang-Ho2
  • Hwang, Mi-Hye2
  • Kim, Tae-Hwan1
  • Kwon, Oh-Deog1
  • Kwak, Dongmi5
  • 1 College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. , (North Korea)
  • 2 Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea. , (North Korea)
  • 3 College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea. , (North Korea)
  • 4 College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. , (North Korea)
  • 5 College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea; Cardiovascular Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: [email protected] , (North Korea)
Type
Published Article
Journal
Parasitology international
Publication Date
Oct 01, 2020
Volume
78
Pages
102154–102154
Identifiers
DOI: 10.1016/j.parint.2020.102154
PMID: 32531468
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Giardia duodenalis (syn. G. intestinalis, G. lamblia) is an important zoonotic parasite infecting livestock (including pigs) through ingesting cysts in contaminated food or water. This parasite has been classified into eight different genetic assemblages, A to H. Here, we examined the individual-level prevalence of G. duodenalis in domestic pig farms and confirmed host specificity by genotype comparisons. Samples were collected from southern and central Korea, between May 2017 and January 2019. DNA directly extracted from 745 pig fecal specimens were tested by PCR for G. duodenalis small subunit ribosomal RNA (ssu rRNA), glutamate dehydrogenase (gdh), and β-giardin gene sequences. Based on ssu rRNA PCR, 110 (14.8%) were positive for G. duodenalis. Infection risk was the highest in the fattener group (31/139, 22.3%) and during the autumn season (52/245, 21.2%: p < .001). No statistically significant differences in risk for infection were observed between fecal types (normal versus diarrheal). Fifty ssu rRNA samples, three gdh samples, and five β-giardin samples were successfully sequenced and genotyped. Ssu rRNA assemblage sequence analysis identified E (40.0%, 20/50), D (34.0%, 17/50), C (24.0%, 12/50), and A (2.0%, 1/50). The gdh locus identified three samples as assemblage E, and the β-giardin locus identified four samples as assemblage E and one as assemblage C. Assemblage A sequences obtained (ssu rRNA; MK430919) had 100% identity with Giardia sequences isolated from a Korean individual (AJ293301), indicating the potential of zoonotic transmission. Continuous management and monitoring for prevention of transmission and protection of animal and human health are essential. Copyright © 2020 Elsevier B.V. All rights reserved.

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