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Multienzyme mimetic activities of holey [email protected] for visual colorimetric and ultrasensitive fluorometric discriminative detection of glutathione and glucose in physiological fluids.

Authors
  • Tang, Weiyang1
  • An, Yena2
  • Chen, Jia3
  • Row, Kyung Ho4
  • 1 School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China; Department of Chemistry and Chemical Engineering, Education and Research Center for Smart Energy and Materials, Inha University, Incheon, 402-701, South Korea. Electronic address: [email protected] , (China)
  • 2 Department of Chemistry and Chemical Engineering, Education and Research Center for Smart Energy and Materials, Inha University, Incheon, 402-701, South Korea. , (North Korea)
  • 3 CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China. Electronic address: [email protected] , (China)
  • 4 Department of Chemistry and Chemical Engineering, Education and Research Center for Smart Energy and Materials, Inha University, Incheon, 402-701, South Korea. Electronic address: [email protected] , (North Korea)
Type
Published Article
Journal
Talanta
Publication Date
May 01, 2022
Volume
241
Pages
123221–123221
Identifiers
DOI: 10.1016/j.talanta.2022.123221
PMID: 35063877
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nanozymes with multiple activities have drawn immense interest owing to their great prospect in biochemical analysis. Fabricating nanomaterials-based artificial enzymes for multiple-enzyme mimetic activity is a significant challenge. This paper reports a sensitive biosensing platform to mimic the peroxidase, oxidase, and catalase-like activity by bimetallic CuPd embedded holey carbon nitride ([email protected]). Owing to the combination of porous H-C3N4 and bimetallic CuPd nanoparticles, the [email protected] exhibited a large specific surface area, extremely high mobility and catalytic activity of electrons, resulting in remarkable triple-enzyme mimetic activity. Owing to the excellent oxidase/peroxidase-like activities of [email protected], a visual colorimetric and ultrasensitive fluorometric biosensing platform was established for the discriminatory detection of glutathione (linear range: 2-40 μM) and glucose (linear range: 0.1-40 μM) in physiological fluids, respectively. The fluorescence detection system showed ultrahigh sensitivity toward H2O2, with a linear range of 30-1500 nM. In addition, a one-step glucose detection strategy was proposed to replace the traditional, complicated two-step detection method, which simplifies the operation steps and improves the detection efficiency. The assay presented in this paper offers an effective multiple-enzymes mimicking detection platform that broaden its promising applications in biomedicine analysis and monitoring. Copyright © 2022 Elsevier B.V. All rights reserved.

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