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Multicontrast nonlinear optical microscopy with a compact and rapid pulse shaper.

Authors
Type
Published Article
Journal
Optics Letters
1539-4794
Publisher
The Optical Society
Publication Date
Volume
37
Issue
13
Pages
2763–2765
Identifiers
DOI: 10.1364/OL.37.002763
PMID: 22743521
Source
Medline

Abstract

Homodyne detection can dramatically enhance measurement sensitivity for weak signals. In nonlinear optical microscopy it can make accessible a range of novel, intrinsic, contrast like nonlinear absorption and nonlinear phase contrast. Here a compact and rapid pulse shaper is developed, implemented, and demonstrated for homodyne detection in nonlinear microscopy with high-repetition rate mode-locked femtosecond lasers. With this method we generate two-photon absorption (TPA) and self-phase modulation images of gold nanostars in biological samples. Simultaneous imaging of two-photon luminescence and TPA also enables us to produce two-photon quantum yield images.

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