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Multichannel separation device with parallel electrochemical detection.

Authors
  • Komendová, Martina1
  • Nawada, Suhas2
  • Metelka, Radovan3
  • Schoenmakers, Peter J2
  • Urban, Jiří4
  • 1 Masaryk University, Department of Chemistry, Brno, Czech Republic. , (Czechia)
  • 2 University of Amsterdam, Van't Hoff Institute for Molecular Sciences, Amsterdam, the Netherlands. , (Netherlands)
  • 3 University of Pardubice, Department of Analytical Chemistry, Pardubice, Czech Republic. , (Czechia)
  • 4 Masaryk University, Department of Chemistry, Brno, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Sep 09, 2019
Pages
460537–460537
Identifiers
DOI: 10.1016/j.chroma.2019.460537
PMID: 31537305
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device. Copyright © 2019 Elsevier B.V. All rights reserved.

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