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Multicenter evaluation of the RAPIDEC® CARBA NP test for rapid screening of carbapenemase-producing Enterobacteriaceae and Gram-negative nonfermenters from clinical specimens.

Authors
  • Coppi, Marco1
  • Antonelli, Alberto1
  • Giani, Tommaso2
  • Spanu, Teresa3
  • Liotti, Flora Marzia3
  • Fontana, Carla4
  • Mirandola, Walter5
  • Gargiulo, Raffaele6
  • Barozzi, Agostino6
  • Mauri, Carola7
  • Principe, Luigi7
  • Rossolini, Gian Maria8
  • 1 Department of Experimental and Clinical Medicine, University of Florence, Italy. , (Italy)
  • 2 Department of Medical Biotechnologies, University of Siena, Siena, Italy. , (Italy)
  • 3 Institute of Microbiology, Catholic University of the Sacred Heart, Agostino Gemelli Hospital, Rome, Italy. , (Italy)
  • 4 Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy; Clinical Microbiology Laboratories, Polyclinic of Tor Vergata Foundation, Rome, Italy. , (Italy)
  • 5 Clinical Microbiology Laboratories, Polyclinic of Tor Vergata Foundation, Rome, Italy. , (Italy)
  • 6 Provincial Laboratory of Clinical Microbiology, S. Agostino-Estense Hospital, Modena, Italy. , (Italy)
  • 7 Microbiology and Virology Unit, A. Manzoni Hospital, Lecco, Italy. , (Italy)
  • 8 Department of Experimental and Clinical Medicine, University of Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy. Electronic address: [email protected] , (Italy)
Type
Published Article
Journal
Diagnostic microbiology and infectious disease
Publication Date
Jul 01, 2017
Volume
88
Issue
3
Pages
207–213
Identifiers
DOI: 10.1016/j.diagmicrobio.2017.04.009
PMID: 28502395
Source
Medline
Keywords
License
Unknown

Abstract

The rapid diagnosis of carbapenemase-producing (CP) bacteria is essential for the management of therapy and infection control. In this study, RAPIDEC® CARBA NP (RCNP) was evaluated for the rapid screening of CP Enterobacteriaceae, Acinetobacter baumannii complex, and Pseudomonas aeruginosa from clinical specimens collected at five Italian hospitals. Firstly, each site tested 20 well-characterized strains in a blinded fashion. Secondly, each center prospectively tested 25 isolates from blood cultures processed with a rapid workflow (6h after subculture) and 25 isolates from other specimens processed after an overnight culture. The presence of carbapenemases was confirmed by multiplex real-timePCRs targeting carbapenemase genes. RCNP presented an overall sensitivity, specificity, positive predictive value, and negative predictive value of 70%, 94%, 82%, and 89%, respectively, with a higher performance in detection of CP Enterobacteriaceae and a poorer performance in detection of CP A. baumannii complex. With isolates from blood cultures, RCNP could significantly reduce the time required for identification of CP Enterobacteriaceae (less than 9h since the positivization of blood cultures).

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