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A multi-colour/multi-affinity marker set to visualize phosphoinositide dynamics in Arabidopsis.

Authors
  • Simon, Mathilde Laetitia Audrey
  • Platre, Matthieu Pierre
  • Assil, Sonia
  • van Wijk, Ringo
  • Chen, William Yawei
  • Chory, Joanne
  • Dreux, Marlène
  • Munnik, Teun
  • Jaillais, Yvon
Type
Published Article
Journal
The Plant Journal
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jan 01, 2014
Volume
77
Issue
2
Pages
322–337
Identifiers
DOI: 10.1111/tpj.12358
PMID: 24147788
Source
Medline
Keywords
License
Unknown

Abstract

Phosphatidylinositolphosphates (PIPs) are phospholipids that contain a phosphorylated inositol head group. PIPs represent a minor fraction of total phospholipids, but are involved in many regulatory processes, such as cell signalling and intracellular trafficking. Membrane compartments are enriched or depleted in specific PIPs, providing a unique composition for these compartments and contributing to their identity. The precise subcellular localization and dynamics of most PIP species is not fully understood in plants. Here, we designed genetically encoded biosensors with distinct relative affinities and expressed them stably in Arabidopsis thaliana. Analysis of this multi-affinity 'PIPline' marker set revealed previously unrecognized localization of various PIPs in root epidermis. Notably, we found that PI(4,5)P2 is able to localize PIP2 -interacting protein domains to the plasma membrane in non-stressed root epidermal cells. Our analysis further revealed that there is a gradient of PI4P, with the highest concentration at the plasma membrane, intermediate concentration in post-Golgi/endosomal compartments, and the lowest concentration in the Golgi. Finally, we also found a similar gradient of PI3P from high in late endosomes to low in the tonoplast. Our library extends the range of available PIP biosensors, and will allow rapid progress in our understanding of PIP dynamics in plants.

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