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Motile sperm recovery from fresh and frozen-thawed ejaculates using a swim-up procedure.

Authors
  • Graczykowski, J W
  • Siegel, M S
Type
Published Article
Journal
Fertility and Sterility
Publisher
Elsevier
Publication Date
Apr 01, 1991
Volume
55
Issue
4
Pages
841–843
Identifiers
PMID: 2010014
Source
Medline
License
Unknown

Abstract

The recovery of motile sperm in swim-ups from fresh semen that was washed and centrifuged one time was 33% and after 2 washes and centrifugations was 22%. The recovery rates for frozen-thawed semen were only 7% and 5%, respectively. The straight line velocity of sperm was increased after sperm swim-up, whereas changes to the lateral sperm head movement were not as evident. However, the dramatic loss in motile sperm numbers especially after swim-up from thawed semen indicate that more efficient methods of isolating motile sperm from thawed semen may be required.

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