Affordable Access

Morphometric characteristics of peroxisomes rats with chronic renal failure induced by five-sixth nephrectomy

Authors
  • De Craemer, Dirk
  • Verbeelen, Dierik
  • Van den Branden, Christiane
Publication Date
Jan 01, 1997
Source
Ghent University Institutional Archive
Keywords
Language
English
License
Unknown
External links

Abstract

An increased H2O2 production and a decreased activity of several peroxisomal oxidases have previously been reported in kidneys of rats with five-sixth nephrectomy, a model for chronic renal failure. We investigated the morphological and morphometric characteristics of peroxisomes, the organelles in which an important part of cellular H2O2 metabolism is localized, in remnant kidneys 16 weeks after operation. The vast majority of renal peroxisomes were found in the epithelial cells of proximal tubules. The organelles were distributed throughout the cells. We observed a significant increase in size, perimeter and volume density of the peroxisomes as compared to normal kidneys. Elongated peroxisomes were less frequent. An inverse linear correlation between mean size and number of peroxisomes was found. In cortex homogenates, the activity of catalase, the peroxisomal H2O2-scavenging enzyme, was significantly decreased and was inversely proportional to the mean peroxisomal diameter. The observed morphological adaptations are believed to create an unfavorable situation for the enzymatic activities in remnant kidney peroxisomes.

Report this publication

Statistics

Seen <100 times