The aim of the study was to investigate the locations, diameters, and connections of the periorbital arterial vessels. The peripheral and marginal arcades of the superior and inferior eyelids were revealed by bilateral meticulous anatomic dissections in 12 adult male and 5 female preserved cadavers. Silicone rubber injection was used to fill the regional arteries. The mean external diameter of the lacrimal (0.4 mm), supraorbital (1.0 mm), supratrochlear (0.9 mm), superior medial palpebral (0.9 mm), inferior medial palpebral (1.0 mm), dorsal nasal (0.8 mm), and infraorbital (2.0 mm) arteries were measured. Contribution of superficial temporal artery to palpebral arcades via frontal, zygomatico-orbital, and transverse facial branches was demonstrated. The purpose of this study was to demonstrate the periorbital arterial supply with contributing anastomosis from facial vasculature and impact on clinical practice.