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Morphological changes in aging cell lines of Paramecium aurelia. III. The effects of emetine on polysome formation.

Authors
  • Sundararaman, V
  • Cummings, D J
Type
Published Article
Journal
Mechanisms of Ageing and Development
Publisher
Elsevier
Publication Date
Jan 01, 1977
Volume
6
Issue
6
Pages
393–406
Identifiers
PMID: 926865
Source
Medline
License
Unknown

Abstract

The effect of emetine, an inhibitor of cellular protein synthesis, on young and old cell lines of Paramecium aurelia was studied. Emetine treatment resulted in the accumulation of ribosomal aggregates in the form of helices or rosettes. In treated young cells, large patches of endoplasmic reticulum with clusters of ribosomes were frequently observed. These structures were smaller in treated aging cells. Owing to the association of these ribosomal clusters with endoplasmic structures, they were called "ribosomal complexes". In young cells the polysome helices were more numerous, compared to those of old cells. The difference in the number of polysomes between the young and old cells appeared to be related to the number of ribosomes. The results suggested that in aging cells there were fewer ribosomes and less RNA. In very old cells the ribosomal particles appeared to be altered morphologically.

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