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Monolithic capillary column with an integrated electrochemical detector.

Authors
  • Komendová, Martina1
  • Metelka, Radovan1
  • Urban, Jiří2
  • 1 Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic. , (Czechia)
  • 2 Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic; Department of Chemistry, Faculty of Science, Masaryk University, 625 00, Brno, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Aug 04, 2017
Volume
1509
Pages
171–175
Identifiers
DOI: 10.1016/j.chroma.2017.06.057
PMID: 28647148
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0mgL-1 and an instrumental limit of detection as low as 24pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74±0.03mgL-1 and 0.71±0.02mgL-1, respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days. Copyright © 2017 Elsevier B.V. All rights reserved.

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