Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis

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Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis

Publisher
American Society for Clinical Investigation
Publication Date
Jan 01, 1999
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

STANDARD OPERATING PROCEDURE http://proteomics.cancer.gov R-119.1 Page 1 of 6 STANDARD OPERATING PROCEDURE Title: Antibody Production at Strategic Diagnostics Inc. SOP#: M-119 Version #: 1 Author: Strategic Diagnostic Inc. Date Approved: August 6, 2009 Date Modified: 1. This procedure is to describe the methods used by Strategic Diagnostic Inc. (SDI) to produce and grow up monoclonal antibodies for the Clinical Proteomic Technologies for Cancer (CPTC) program. PURPOSE 2. This document describes the procedure for production and growth of monoclonal antibodies to specific cancer related target proteins. SCOPE 3. It is the responsibility of the person(s) preparing these reagents to be familiar with lab safety procedures and to have basic laboratory skills. RESPONSIBILITIES 4. • Motic Microscope, Model AE30 EQUIPMENT • Reichert Bright Line Hemacytometer (0.1mm deep) • Thermo Scientific Centrifuge, Model FL40R • Thermo Scientific Shaker, Model 2314 • Thermo Fisher Incubator. Model Hera Cell 240 • SpectraMax M5 Plate Reader • CryoMed Liquid Nitrogen Tank, Model 1520HE • Puffer Hubbard -70°C Freezer, Model 10F8517 5. • Swiss Webster Mice (Taconic ) MATERIALS • Complete Freunds Adjuvant (Sigma, Cat. #F5881) http://proteomics.cancer.gov R-119.1 Page 2 of 6 • Incomplete Freunds Adjuvant (Sigma, Cat. #F5506) • Sterile instruments (forceps, scissors and/or tweezers) • Strainer (Fisher Scientific, Cat. #22-363-547) • Iscoves Modified Dulbeccos Medium (Media Tech, Cat. #10-016-CM) • Hypoxanthine Aminopterin Thymidine (Invitrogen, Cat. #31062) • Hypoxanthine Thymidine (Invitrogen, Cat. #11067030) • Polyethylene Glycol (Roche, Cat. #0783641001) • P3x63Ag8.653 cells • ELISA plates (Fisher Scientific, Cat. #21-377-270) • 96 well TC Plates (Fisher Scientific, Cat. #07-200-91) • Cryovials (Fisher Cat. #03-337-7W) • TMB (Strategic Diagnostics, Cat. #1026218

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