A monoclonal antibody specific for Pseudomonas aeruginosa amidase.
- Published Article
- Publication Date
Aug 01, 2001
Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/11604114