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A monoclonal antibody specific for Pseudomonas aeruginosa amidase.

Authors
  • Novo, C
  • Karmali, A
  • Clemente, A
  • Brown, P R
Type
Published Article
Journal
Hybridoma
Publication Date
Aug 01, 2001
Volume
20
Issue
4
Pages
273–279
Identifiers
PMID: 11604114
Source
Medline
License
Unknown

Abstract

Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.

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