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Monoclonal antibodies of predetermined specificity for fibrin: a rational approach to monoclonal antibody production.

Authors
Type
Published Article
Journal
Hybridoma
Publication Date
Volume
5
Issue
3
Pages
215–222
Identifiers
PMID: 2429911
Source
Medline

Abstract

Three monoclonal antibodies (MAbs) (55D10, 59D8, and 64C5) have been produced by an immunization strategy that uses a synthetic heptapeptide from the amino terminus of the beta-chain in human fibrin. These MAbs bind to human fibrin in the presence of 4 mg/ml human fibrinogen in solid-phase radioimmunoassay. Ligand specificity studies demonstrate that antifibrin immunoreactivity is completely inhibited by a series of synthetic fibrin-like beta-peptides that bear the position-2 histidyl residue. Although the presence of this residue on the epitope is necessary for its recognition by a fibrin-specific antibody, the presence of the histidyl residue alone is not sufficient for antibody recognition.

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