Monoclonal antibodies against RNA-dependent DNA polymerase from myeloblastosis-associated viruses.
- Published Article
- Publication Date
Jan 01, 1987
Two lines of hybridomas, RT-12 and RT-14, secreting monoclonal antibodies against the reverse transcriptase from myeloblastosis-associated viruses have been prepared. The monoclonal antibodies RT-12 and RT-14 specifically react with reverse transcriptase, as has been shown by radioimmunoassay and enzyme-linked immunoassay techniques after SDS-PAGE and blotting to nitrocellulose membranes. It has been shown that antigenic determinants for RT-12 and RT-14 are stable to SDS denaturation, hence they belong to a linear type; they are located on the alpha subunit of the enzyme.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/2439388