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Monitoring specific interaction of low molecular weight biomolecules on oxidized porous silicon using ellipsometry.

Authors
  • van Noort, D1
  • Welin-Klintström, S
  • Arwin, H
  • Zangooie, S
  • Lundström, I
  • Mandenius, C F
  • 1 Department of Physics and Measurement Technology, Linköping University, Sweden. , (Sweden)
Type
Published Article
Journal
Biosensors and Bioelectronics
Publisher
Elsevier
Publication Date
Mar 01, 1998
Volume
13
Issue
3-4
Pages
439–449
Identifiers
PMID: 9642775
Source
Medline
License
Unknown

Abstract

Porous silicon dioxide surfaces have been used for monitoring the specific affinity binding of low molecular weight molecules to streptavidin. Streptavidin was immobilized to the porous silicon dioxide surface by spontaneous adsorption at pH 7.4. Binding of biotin and an oligopeptide synthesized by means of combinatorial chemistry were monitored with an in situ null ellipsometer. Measurements were also done with hydroxy-azobenzene-2-carboxylic acid and DL-6-8-thioctic acid amide. The performance of porous silicon dioxide as a potential surface in biosensor applications was compared with a planar silicon dioxide surface. Porous silicon dioxide showed a 10-fold amplification of the response compared to planar silicon dioxide. It was possible to monitor the binding of biotin and the oligopeptide in the concentration range 2-40 microM. A response time as low as 30 s was obtained for the oligopeptide at 40 microM.

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