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Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.

Authors
  • Venkateswaran, K S
  • Raghuveeran, C D
  • Gopalan, N
  • Agarwal, G S
  • Kaushik, M P
  • Vijayaraghavan, R
Type
Published Article
Journal
Biochemistry international
Publication Date
Dec 01, 1992
Volume
28
Issue
4
Pages
745–750
Identifiers
PMID: 1482410
Source
Medline
License
Unknown

Abstract

Carbamylation of haemoglobin by methyl isocyanate (MIC) was detected by high-performance liquid chromatography (HPLC) using a photodiode array detector following cyclisation of the N-terminal valine into methyl isopropyl hydantoin (MIH). MIH was also synthesised by reaction of MIC with valine, the chromatographic conditions standardised and the spectrum derived by a photodiode array detector recorded for confirmation of the identity of MIH. This HPLC method is specific, sensitive and suitable for the detection of exposure of blood samples to methyl isocyanate.

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