Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.
- Published Article
- Publication Date
Dec 01, 1992
Carbamylation of haemoglobin by methyl isocyanate (MIC) was detected by high-performance liquid chromatography (HPLC) using a photodiode array detector following cyclisation of the N-terminal valine into methyl isopropyl hydantoin (MIH). MIH was also synthesised by reaction of MIC with valine, the chromatographic conditions standardised and the spectrum derived by a photodiode array detector recorded for confirmation of the identity of MIH. This HPLC method is specific, sensitive and suitable for the detection of exposure of blood samples to methyl isocyanate.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/1482410