Affordable Access

deepdyve-link
Publisher Website

Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism.

Authors
  • Petroianu, Georg1
  • Szoke, Eva
  • Kalász, Huba
  • Szegi, Péter
  • Laufer, Rudolf
  • Benko, Bernadett
  • Darvas, Ferenc
  • Tekes, Kornélia
  • 1 Department of Cell Biology, Florida International University, Miami, FL, USA.
Type
Published Article
Journal
The Open Medicinal Chemistry Journal
Publisher
Bentham Science
Publication Date
Jul 29, 2009
Volume
3
Pages
1–7
Identifiers
DOI: 10.2174/1874104500903010001
PMID: 19707521
Source
Medline
Keywords
License
Unknown

Abstract

Three major flavonoid chamomile components (quercetin, apigenin-7-O-glucoside and rutin) were subjected to oxidative metabolism by cytochrome P-450 of rat liver microsomal preparations. Changes over time in their respective concentrations were followed using reversed-phase HPLC with UV detection. No clean-up had to be applied as only the specific flavonoid had to be separated from the background components originating from the rat liver microsome.Neither the concentration of apigenin-7-O-glucoside nor that of the diglycoside rutin decreased during one hour of exposure to rat microsomal treatment. In contrast, the concentration of quercetin, a lipophilic aglycon, decreased.Our analytical HPLC results complement the in silico calculated lipophilicity (logP) of these compounds; the relatively high lipophilicity of quercetin appears to predispose it to oxidative metabolism in order to decrease its fat solubility. In contrast the much less lipophilic compounds apigenin-7-O-glucoside and rutin were resistant in vitro to microsomal treatment.

Report this publication

Statistics

Seen <100 times